منابع مشابه
Potential applications of bacteriorhodopsin mutants.
Bacteriorhodopsin (BR), a model system in biotechnology, is a G-protein dependent trans membrane protein which serves as a light driven proton pump in the cell membrane of Halobacterium salinarum. Due to the linkage of retinal to the protein, it seems colored and has numbers of versatile properties. As in vitro culture of the Halobacteria is very difficult, and isolation is time consuming and u...
متن کاملUnravelling the folding of bacteriorhodopsin.
The folding mechanism of integral membrane proteins has eluded detailed study, largely as a result of the inherent difficulties in folding these proteins in vitro. The seven-transmembrane helical protein bacteriorhodopsin has, however, allowed major advances to be made, not just on the folding of this particular protein, but also on the factors governing folding of transmembrane alpha-helical p...
متن کاملColor recognition with bacteriorhodopsin.
We have studied opto-electric properties of wild type bacteriorhodopsin and its two artificial variants. We have measured opto-electric responses with respect to wavelength for all three proteins and we describe the use of the proteins for color detection. Opto-electric responses of proteins to set of colored lights were measured and it has been shown that bacteriorhodopsin and its variants can...
متن کاملDeprotonation of lipid-depleted bacteriorhodopsin.
The removal of 75% of the lipid from bacteriorhodopsin caused the following: (i) decreased efficiency and rate of deprotonation of the protonated Schiff base (as monitored by absorption of the M412 intermediate); (ii) increased efficiency of deprotonation of deionized samples; (iii) a decrease by 1 unit in the pH at which deprotonation ceases; (iv) increased intensity of Eu3+ emission in Eu3+-r...
متن کاملBacteriorhodopsin optoelectronic synapses.
Synapses are critical components of an artificial neural network. Bacteriorhodopsin thin film can be used to construct compact, finely graded synapses for an optoelectronic neural network, based on its photochromic properties. Measurements show that these photochromic changes are blocked at low temperature. Thermal gating will allow synapses to be written on the film optically and then read wit...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1991
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(91)82042-2